Accelerate PCR genotyping: amplify crude sample without DNA extraction using SampleIN Direct PCR Kit

SampleIN™ Direct PCR Kit is the great tool to perform fast PCR without template DNA extraction

Product	Specialties	ALLin™ buffer with dNTPs	Fast cycling	GC/AT rich PCR	Max product	High Yields	Direct PCR	Multiplex PCR	Direct gel loading
SampleIN™ Direct PCR Kit	Fast, direct crude sample PCR, includes ALLin™ HS Taq DNA Polymerase in 2X mastermix with red loading dye (available separately)	●	●	●	5 kb	●	●●●	●	●
ALLin™ Taq DNA Polymerase, 5 u/µl	Robust Taq with ALLin™ buffer for standard, fast, GC rich PCR	●	●	●	6 kb	●●	●	●
ALLin™ Red Taq Mastermix, 2X	ALLin™ Taq DNA Polymerase in 2X mastermix with loading dye	●	●	●	6 kb	●●	●	●	●
ALLin™ Taq Mastermix, 2X	ALLin™ Taq DNA Polymerase in 2X mastermix	●	●	●	6 kb	●●	●	●
Taq DNA Polymerase, 5 u/µl	Classical Taq, magnesium supplied separately				5 kb	●

Tips for the best direct PCR or crude sample PCR amplification results:

• If direct PCR amplification does not work, reduce the sample input amount, for example use less of bacterial colony material or less of tissue.

• For some samples you might need performing a simple lysis with proteinase K, lysozyme or commercially available direct PCR amplification lysis buffers.

• Try different dilutions of the lysed sample for the PCR amplification.

• Choose primers with high specificity and efficiency for the target sequence to ensure successful amplification.

• Use only the hot-start DNA polymerase or master mix for the best amplification result when working with crude samples.

• Determine the optimal annealing temperature by conducting a temperature gradient PCR to enhance primer binding and specificity.

• Extend the denaturation step to ensure complete DNA denaturation, especially in crude samples with potentially more inhibitors that can be degraded when heating.

• Include positive control (known DNA template/primers) and negative controls (no template) to validate the PCR amplification results.

Professionally Simple