High-Fidelity PCR

ALLin™ Mega HS HiFi Polymerase - most precise, hot-start, enzyme of choice for NGS applications

Product	Specialties	ALLin™ buffer with dNTPs	Fast cycling	GC/AT rich PCR	Sensitive, hot-start	Proofreading (3’-5’ exo)	Fidelity vs Taq	Max product	Direct PCR	Multiplex PCR	Cloning
ALLin™ Mega HS HiFi DNA Polymerase, 2 u/µl	Hot start version of ALLin™ Mega HiFi Polymerase: 100X higher fidelity than Taq for fast, complex, long PCR, excellent for NGS	●	●●	●●●	●	●●●	~100X	>20 kb	●	●●●	Blunt
ALLin™ Mega HS HiFi Mastermix, 2X	ALLin™ Mega HS HiFi Polymerase in 2X mastermix format	●	●●	●●●	●	●●●	~100X	>20 kb	●	●●●	Blunt
ALLin™ Mega HS HiFi Red Mastermix, 2X	ALLin™ Mega HS HiFi Polymerase in 2X ready-to-load red mastermix format	●	●●	●●●	●	●●●	~100X	>20 kb	●	●●●	Blunt
ALLin™ Mega HiFi DNA Polymerase, 2 u/µl	100X higher fidelity than Taq for a very fast, complex, long PCR, excellent for NGS	●	●●	●●●		●●●	~100X	>20 kb	●	●	Blunt
ALLin™ Mega HiFi Mastermix, 2X	ALLin™ Mega HiFi Polymerase in 2X mastermix format	●	●●	●●●		●●●	~100X	>20 kb	●	●	Blunt
ALLin™ Mega HiFi Red Mastermix, 2X	ALLin™ Mega HiFi Polymerase in 2X ready-to-load red mastermix format	●	●●	●●●		●●●	~100X	>20 kb	●	●	Blunt
ALLin™ HiFi DNA Polymerase, 2 u/µl	50X higher fidelity than Taq for Fast, GC rich, direct PCR with high fidelity	●	●	●●		●●	~50X	10 kb	●		Blunt
ALLin™ RPH Polymerase, 5 u/µl	Robust Proofreading Hot-start Polymerase for long hot start PCR	●	●	●●	●	●	~ 5X	35 kb	●●	●●	TA
ALLin™ RPH Mastermix, 2X	ALLin™ RPH Polymerase in 2X mastermix format	●	●	●●	●	●	~ 5X	35 kb	●●	●●	TA

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