Isothermal Amplification Enzymes
phi29 and Bst as well as Hot Start Bst Polymerases for isothermal amplification applications
| Product | Key Applications | Included | Enzyme | Template | 3'-5' exo (proofreading) | Optimal Temperature | Inactivation |
| ALLin™ Bst DNA Polymerase |
Isothermal DNA amplification at up to 70°C, LAMP |
ALLin™ Bst DNA Polymerase, 8 u/µl, 10X Buffer with dNTPs, 5X Bst Enhancer |
Bst DNA Polymerase large fragment | DNA | 65°C | 10 min. at 80°C | |
| ALLin™ Hot Start Bst Polymerase |
Isothermal DNA amplification at up to 70°C, with a hot-start function and ambient temperature reaction setup, LAMP |
ALLin™ Hot Start Bst DNA Polymerase, 8 u/µl, 10X Buffer with dNTPs, 5X Bst Enhancer |
Hot Start Bst DNA Polymerase, large fragment | DNA | 65°C | 10 min. at 80°C | |
| phi29 DNA Polymerase |
Isothermal DNA amplification at 30°C, |
phi29 DNA Polymerase, 10 u/µl and 10X phi29 Buffer. |
Recombinant phi29 DNA Polymerase | DNA | ● | 30°C | 10 min. at 65°C |
| The use of these products in certain applications, some additives or protocols may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases. | |||||||
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