Isothermal Amplification
Enzymes and kits for common isothermal amplification applications

Product	Key Applications	Included	Enzyme	Template	3'-5' exo (proofreading)	Optimal Temperature	Inactivation
ALLin™ Bst DNA Polymerase	Isothermal DNA amplification at up to 70°C, LAMP	ALLin™ Bst DNA Polymerase, 8 u/µl, 10X Buffer with dNTPs, 5X Bst Enhancer	Bst DNA Polymerase large fragment	DNA		65°C	10 minutes at 80°C
ALLin™ Hot Start Bst Polymerase	Isothermal DNA amplification at up to 70°C, with a hot-start function and ambient temperature reaction setup, LAMP	ALLin™ Hot Start Bst DNA Polymerase, 8 u/µl, 10X Buffer with dNTPs, 5X Bst Enhancer	Hot Start Bst DNA Polymerase, large fragment	DNA		65°C	10 minutes at 80°C
ALLin™ HS Isothermal DNA Amplification Kit	Isothermal DNA amplification at up to 70°C, with a hot-start function and ambient temperature reaction setup, LAMP	Bst Mix w. buffer, dNTPs, Bst. Dye for optional real-time detection. Water.	Hot Start Bst DNA Polymerase, large fragment	DNA		65°C	10 minutes at 80°C
ALLin™ HS Iso-Colorimetric DNA Amplification Kit	Isothermal DNA amplification with a colorimetric (visual) target amplification detection, with a hot-start function and ambient temperature reaction setup	Colour-changing ( when amplification occurs) Mix w. buffer, dNTPs, Bst. Water.	Hot Start Bst DNA Polymerase, large fragment	DNA		65°C	10 minutes at 80°C
Isothermal DNA Amplification Kit	Isothermal DNA amplification at 70°C, LAMP	Mix w. buffer, dNTPs, Bst. Dye for optional real-time detection. Water.	Bst DNA Polymerase large fragment	DNA		65°C	10 minutes at 80°C
Isothermal 1Step RNA Amplification Kit	Virus RNA detection, SARS-CoV-2 detection, Isothermal RNA amplification, RT-LAMP	Reverse transcription mix. Mix w. buffer, dNTPs, Bst. Dye for optional real-time detection. Water.	Reverse Transcriptase with RNase Inhibitor. Bst DNA Polymerase large fragment.	RNA		65°C	10 minutes at 80°C
phi29 DNA Polymerase	Isothermal DNA amplification at 30°C, RCA, WGA, MDA	phi29 DNA Polymerase, 10 u/µl and 10X phi29 Buffer.	Recombinant phi29 DNA Polymerase	DNA	●	30°C	10 minutes at 65°C
PCRbeam™ Fast PCR Detection Kit	Fast and sensitive detection of specific amplification products obtained with LAMP, and other isothermal amplification, as well as PCR based methods. The kit includes membrane strips and buffer, is based on immunological reaction driven by Biotin/FITC and applicable to common low throughput amplification product detection workflows.
The use of these products in certain applications, some additives or protocols may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases.

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Isothermal AmplificationEnzymes and kits for common isothermal amplification applications

Isothermal Amplification
Enzymes and kits for common isothermal amplification applications