SecurRIN™ Advanced RNase Inhibitor is a premium tool for RNA protection from degradation during enzymatic reactions, storage or extraction. It is an extraordinary stable and robust enzyme: it works at up to 60°C temperature, remains active after weeks of room temperature exposure and multiple freezing thawing cycles. It is active in different buffer conditions within a broad pH range of 5.5 to 9.0, and within 0.5 - 1 mM concentration of DTT. SecurRIN™ Advanced RNase Inhibitor is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C binding them in a 1 to 1 ratio. It is a recombinant protein derived from E. coli strain carrying human placenta RNase Inhibitor gene. SecurRIN™ Advanced RNase Inhibitor does not inhibit RNAses H, 1, T1, T2 and S1 Nuclease. It influences neither the activity nor the performance of DNA polymerases and of Reverse Transcriptases. The enzyme is free from DNAses and RNAses. Ribonuclease Inhibitor is a protein that protects RNA samples from degradation by ribonucleases. Ribonucleases are present everywhere in laboratory environment, they are very robust and ubiquitous enzymes that rapidly degrade RNA by destroying valuable RNA samples during molecular biology or diagnostics workflows. RNase inhibitor binds to RNases and inactivates these enzymes, ensuring the integrity and stability of RNA samples during such processes as RNA isolation, clean-up, cDNA synthesis, RT-qPCR and other applications. A good RNase inhibitor should have high affinity for RNases and strong inhibitory activity under a wide variety of buffer conditions. It shall tolerate different pH values, salt concentrations and shall have high affinity to different types of RNases. It should also be stable under higher temperatures and easy to use in all common RNA-related buffers with minimal interference with downstream applications. Furthermore, it is especially important, that the Inhibitor itself is pure and free from contaminating RNases. highQu offers SecurRIN™ Advanced RNase Inhibitor for RNA protection from degradation during enzymatic reactions, storage or extraction. Is is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C.  

The SampleIN™ Direct qPCR Probe Mix has been specifically designed for use with unpurified samples or crude lysates as templates. This 4X concentrated qPCR master mix with the Hot Start Taq DNA Polymerase, dNTPs, magnesium, and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates. This Direct qPCR Probe Mix contains all compounds required for robust qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. Mix is supplied with PCR Water.Though the SampleIN™ Direct qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.For work with crude or inhibitor-rich RNA templates, we offer a SampleIN™ 1Step RT qPCR Probe Mixes, 4X with reverse transcriptase. The SampleIN™ Direct qPCR Probe Mix does not include ROX, the version with Low ROX concentration is available as SampleIN™ Direct qPCR Probe ROX L Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.Tolerates common inhibitors, such as:5-7% crude saliva and crude blood in the reactionChemicals left after NA extractions (guanidine, alcohols, SDS) Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)Saliva and urine compounds (urea)Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

SampleIN™ Direct qPCR Probe ROX L Mix has been specifically designed for use with unpurified samples or crude lysates as templates. This 4X concentrated qPCR master mix with the Hot Start Taq DNA Polymerase, dNTPs, magnesium, and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates. This Direct qPCR Probe Mix contains all compounds required for robust qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. Mix is supplied with PCR Water.Though the SampleIN™ Direct qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.For work with crude or inhibitor-rich RNA templates, we offer a SampleIN™ 1Step RT qPCR Probe Mixes, 4X with reverse transcriptase. SampleIN™ Direct qPCR Probe ROX L Mix includes ROX at low concentration, the version without ROX is available as SampleIN™ Direct qPCR Probe Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.Tolerates common inhibitors, such as:5-7% crude saliva and crude blood in the reactionChemicals left after NA extractions (guanidine, alcohols, SDS) Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)Saliva and urine compounds (urea)Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

SampleIN™ 1Step RT qPCR Probe Mix has been specifically designed for use with crude lysates and impure templates. This 4X concentrated single-tube RT qPCR master mix with the Hot Start Taq DNA Polymerase, Reverse Transcriptase, RNase inhibitor, dNTPs, magnesium and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct one-step qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates. This 1Step RT qPCR Probe Mix contains all compounds required for robust single-tube RT qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. The Mix is supplied with PCR Water.SampleIN™ 1Step RT qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.For work with crude or inhibitor-rich DNA templates, we offer a SampleIN™ Direct qPCR Probe Mixes. SampleIN™ 1Step RT qPCR Probe Mix does not include ROX, the version with Low ROX concentration is available as SampleIN™ 1Step RT qPCR Probe ROX L Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.Tolerates common inhibitors, such as:5-7% crude saliva and crude blood in the reactionChemicals left after NA extractions (guanidine, alcohols, SDS) Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)Saliva and urine compounds (urea)Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

SampleIN™ 1Step RT qPCR Probe ROX L Mix has been specifically designed for use with crude lysates and impure templates. This 4X concentrated single-tube RT qPCR master mix with the Hot Start Taq DNA Polymerase, Reverse Transcriptase, RNase inhibitor, dNTPs, magnesium and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct one-step qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates. This 1Step RT qPCR Probe Mix contains all compounds required for robust single-tube RT qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. The Mix is supplied with PCR Water.SampleIN™ 1Step RT qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.For work with crude or inhibitor-rich DNA templates, we offer a SampleIN™ Direct qPCR Probe Mixes. SampleIN™ 1Step RT qPCR Probe ROX L Mix  includes ROX at low concentration, the version without ROX is available as SampleIN™ 1Step RT qPCR Probe Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.Tolerates common inhibitors, such as:5-7% crude saliva and crude blood in the reactionChemicals left after NA extractions (guanidine, alcohols, SDS) Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)Saliva and urine compounds (urea)Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.

highQu ALLin™ RPH Polymerase (Robust, Proofreading, Hot-start Polymerase) is the versatile engineered enzyme combining best polymerase properties for excellence in most demanding PCR applications, like low copy detection, long or high fidelity PCR, amplification of complex templates, crude sample PCR and multiplexing. ALLin™ RPH Polymerase has 5 times higher fidelity than Taq DNA Polymerase and produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ RPH Mastermix, 2X is available.

highQu ALLin™ Hot Start Taq DNA Polymerase is the superior sensitive enzyme. The activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of crude, complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Hot Start Taq DNA Polymerase is maximized by the use of 2X Red colored Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with PCR Water, and the only thing to add is the template with primers. ALLin™ HS Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. ALLin™ HS Red Taq Mastermix, 2X is also a key component in highQu SampleIN™ Direct PCR Kit (DPK0101/5), ensuring outstanding PCR results with crude samples.