Isothermal Amplification
Enzymes and kits for common isothermal amplification applications
| Product | Key Applications | Included | Enzyme | Strand Displacement | Template | 3'-5' exo (proofreading) |
5'-3' exo | Optimal Temperature | Inactivation |
| Isothermal DNA Amplification Kit |
Isothermal DNA amplification at 70°C, |
Mix w. buffer, dNTPs, Bst. Dye for optional real-time detection. Water. |
Bst DNA Polymerase large fragment | ● | DNA | 65°C | 10 minutes at 80°C | ||
| Isothermal 1Step RNA Amplification Kit | Virus RNA detection, SARS-CoV-2 detection, Isothermal RNA amplification, RT-LAMP |
Reverse transcription mix. Mix w. buffer, dNTPs, Bst. Dye for optional real-time detection. Water. |
Reverse Transcriptase with RNase Inhibitor. |
● | RNA |
65°C |
10 minutes at 80°C | ||
| phi29 DNA Polymerase |
Isothermal DNA amplification at 30°C, |
phi29 DNA Polymerase, 10 u/µl and 10X phi29 Buffer. |
Recombinant phi29 DNA Polymerase | ● | DNA | ● | 30°C | 10 minutes at 65°C | |
| PCRbeam™ Fast PCR Detection Kit |
Fast and sensitive detection of specific amplification products obtained with LAMP, and other isothermal amplification, as well as PCR based methods. The kit includes membrane strips and buffer, is based on immunological reaction driven by Biotin/FITC and applicable to common low throughput amplification product detection workflows. |
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| The use of these products in certain applications, some additives or protocols may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases. | |||||||||
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