Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions. The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as lysed crude samples can serve as templates for one-step RT qPCR. Though the kit was not specifically designed for crude sample qPCR, it has a potential to work well for this application. The robust 1Step RT qPCR Pro Mix is a 2X concentrated qPCR master mix which includes dNTPs, buffer, and the hot start Taq DNA Polymerase. The Hot Start function ensures the highest sensitivity and specificity under both standard and fast qPCR cycling conditions. The kits provide excellent results on both AT and GC-rich templates and show early Ct values with minimum or no optimization. Our kits include PCR Water to guaranty the best performance. To suit the broad instrument range the 2X 1Step RT qPCR Probe Kits are available in three versions – without ROX, with low or high ROX concentration.

highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band.

The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.

Proteinase K (Molecular Biology Grade Solution) is a serine peptidase with a very high specific activity and a broad spectrum of protein digestion possibilities. The solution is designed to be used for protein degradation (up to tetrapeptides) during the cell lysis and RNA/DNA extraction procedures under hush reaction conditions such as the higher temperatures and the presence of detergents. The enzyme efficiently degrades DNases and RNases during nucleic acid isolation process. The high purity of the Proteinase K and controlled absence of both DNAse and RNase contamination ensures the integrity of nucleic acids. The 100% enzyme activity (when stored at -20°C) is guaranteed for at least two years after production. However, the experiments proved that the proteinase remains close to 90% active even when stored at +37°C for 18 months. The enzyme is supplied as a 20 mg/ml concentrated solution with an average specific activity of more than 800 u/ml. Active in all common buffers used for cell lysis and RNA/DNA extraction, in a presence of urea, SDS and guanidinium salts Stable at high temperature of up to 56°C Can be inactivated by heating at 65oC for 20 minutes or at 75oC for 10 minutes Active in a pH range of 4–12 with an optimum pH 7.5–8.0 The Proteinase K gene from album expressed in yeast host. The quality limit of allowed host DNA presence is ≤ 0.25 pg/U measured by qPCR what is about a half when compared to other suppliers. Unit Definition Folin & Ciocalteu’s method - One unit is required to hydrolyze urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine in 1 minute at 37°C and pH 7.5, 1 U = 1 m Anson U.

highQu Synthetic Carrier RNA is designed to be used in all kind nucleic acid purification and precipitation procedures as a carrier and co-precipitant of nucleic acids. It is especially useful to increase the amount of RNA or DNA pellet in low concentrated solutions, in such procedures, as viral RNA extraction from human specimen samples. In contrast to commonly used carrier RNAs such as tRNA, yeast RNA, or sonicated salmon sperm DNA, the Synthetic Carrier RNA is free from animal or yeast RNA contamination. Coprecipitated RNA and DNA can be directly used for all common downstream applications, such as PCR or RT-PCR, as well as highly sensitive qPCR. The use of carrier RNAs for coprecipitation of nucleic acids may interfere with spectrophotometrical concentration measurements. The presence of carrier RNAs in the RNA or DNA solution may have some influence on certain enzymatic reactions performed by such enzymes that act on all nucleic acid molecules, for example T4 Polynucleotide Kinase or Terminal DNA Transferase.

highQu PCR Water is a supplementary high quality reagent for all demanding PCR and qPCR and other molecular biology applications. It saves time being on your bench and guaranties the purity of reactions and inhibition-free performance of PCR reagents. highQu PCR Water is a deionized, membrane filtered water continuously tested in ultrasensitive qPCR and PCR applications, in amplification of long targets and highly specific detection of few copies of templates.