highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors. ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.

The SampleIN™ Direct qPCR Probe Mix has been specifically designed for use with unpurified samples or crude lysates as templates. This 4X concentrated qPCR master mix with the Hot Start Taq DNA Polymerase, dNTPs, magnesium, and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates. This Direct qPCR Probe Mix contains all compounds required for robust qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. Mix is supplied with PCR Water.Though the SampleIN™ Direct qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.For work with crude or inhibitor-rich RNA templates, we offer a SampleIN™ 1Step RT qPCR Probe Mixes, 4X with reverse transcriptase. The SampleIN™ Direct qPCR Probe Mix does not include ROX, the version with Low ROX concentration is available as SampleIN™ Direct qPCR Probe ROX L Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.Tolerates common inhibitors, such as:5-7% crude saliva and crude blood in the reactionChemicals left after NA extractions (guanidine, alcohols, SDS) Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)Saliva and urine compounds (urea)Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

SampleIN™ Direct qPCR Probe ROX L Mix has been specifically designed for use with unpurified samples or crude lysates as templates. This 4X concentrated qPCR master mix with the Hot Start Taq DNA Polymerase, dNTPs, magnesium, and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates. This Direct qPCR Probe Mix contains all compounds required for robust qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. Mix is supplied with PCR Water.Though the SampleIN™ Direct qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.For work with crude or inhibitor-rich RNA templates, we offer a SampleIN™ 1Step RT qPCR Probe Mixes, 4X with reverse transcriptase. SampleIN™ Direct qPCR Probe ROX L Mix includes ROX at low concentration, the version without ROX is available as SampleIN™ Direct qPCR Probe Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.Tolerates common inhibitors, such as:5-7% crude saliva and crude blood in the reactionChemicals left after NA extractions (guanidine, alcohols, SDS) Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)Saliva and urine compounds (urea)Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

SampleIN™ 1Step RT qPCR Probe Mix has been specifically designed for use with crude lysates and impure templates. This 4X concentrated single-tube RT qPCR master mix with the Hot Start Taq DNA Polymerase, Reverse Transcriptase, RNase inhibitor, dNTPs, magnesium and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct one-step qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates. This 1Step RT qPCR Probe Mix contains all compounds required for robust single-tube RT qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. The Mix is supplied with PCR Water.SampleIN™ 1Step RT qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.For work with crude or inhibitor-rich DNA templates, we offer a SampleIN™ Direct qPCR Probe Mixes. SampleIN™ 1Step RT qPCR Probe Mix does not include ROX, the version with Low ROX concentration is available as SampleIN™ 1Step RT qPCR Probe ROX L Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.Tolerates common inhibitors, such as:5-7% crude saliva and crude blood in the reactionChemicals left after NA extractions (guanidine, alcohols, SDS) Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)Saliva and urine compounds (urea)Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

SampleIN™ 1Step RT qPCR Probe ROX L Mix has been specifically designed for use with crude lysates and impure templates. This 4X concentrated single-tube RT qPCR master mix with the Hot Start Taq DNA Polymerase, Reverse Transcriptase, RNase inhibitor, dNTPs, magnesium and optimized buffer delivers an exceptional PCR inhibitor tolerance in direct one-step qPCR applications. It includes PCR enhancers and stabilizers and is formulated to provide the robust performance with such common sample materials like unpurified saliva or fresh blood. The mix tolerates a range of common chemicals present in purified DNA templates such as guanidine, alcohols, SDS and similar, as well as common blood, urine and environmental natural sample-compounds known to inhibit PCR such as hemoglobin, immunoglobulins, heparin, urea, polyphenols, cellulose, humic and tannic acids and chlorophyl.The mix can be used with all probe types and with a variety of sample amounts from a very low-copy number targets in diluted samples to abundant templates. This 1Step RT qPCR Probe Mix contains all compounds required for robust single-tube RT qPCR reaction, and the only components to add are template, primers and probes. The high concentration of the master mix enables the use of maximal template volume as well as multiple probes and primers. The Mix is supplied with PCR Water.SampleIN™ 1Step RT qPCR Probe Mix has been successfully tested for the use with fresh blood and urine samples; for more consistent results, it is always recommended to purify the template, or at least to perform fast lysis using highQu SampleIN™ Lysis Set for PCR/qPCR.For work with crude or inhibitor-rich DNA templates, we offer a SampleIN™ Direct qPCR Probe Mixes. SampleIN™ 1Step RT qPCR Probe ROX L Mix  includes ROX at low concentration, the version without ROX is available as SampleIN™ 1Step RT qPCR Probe Mix. If high ROX concentration or more ROX flexibility is required, ROX for qPCR Mixes, 50 μM can be obtained separately and added directly into the qPCR mix.Tolerates common inhibitors, such as:5-7% crude saliva and crude blood in the reactionChemicals left after NA extractions (guanidine, alcohols, SDS) Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)Saliva and urine compounds (urea)Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.

highQu ALLin™ RPH Polymerase (Robust, Proofreading, Hot-start Polymerase) is the versatile engineered enzyme combining best polymerase properties for excellence in most demanding PCR applications, like low copy detection, long or high fidelity PCR, amplification of complex templates, crude sample PCR and multiplexing. ALLin™ RPH Polymerase has 5 times higher fidelity than Taq DNA Polymerase and produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ RPH Mastermix, 2X is available.

Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions. The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as lysed crude samples can serve as templates for one-step RT qPCR. Though the kit was not specifically designed for crude sample qPCR, it has a potential to work well for this application. The robust 1Step RT qPCR Pro Mix is a 2X concentrated qPCR master mix which includes dNTPs, buffer, and the hot start Taq DNA Polymerase. The Hot Start function ensures the highest sensitivity and specificity under both standard and fast qPCR cycling conditions. The kits provide excellent results on both AT and GC-rich templates and show early Ct values with minimum or no optimization. Our kits include PCR Water to guaranty the best performance. To suit the broad instrument range the 2X 1Step RT qPCR Probe Kits are available in three versions – without ROX, with low or high ROX concentration.