ALLin™ Hot Start Taq Polymerase

Applications

  • Hot-start PCR up to 6 kb, Low copy target detection.
  • PCR of complex (GC rich) templates.
  • Fast PCR, multiplexing, TA cloning.

Benefits

  • Higher yields, no background in standard or fast PCR.
  • Success on longer (6 kb) or GC rich templates, in crude sample PCR.
  • 5X ALLin™ PCR Buffer contains optimal Mg2+ and dNTPs.

€299.50*

Product sizes
500 units
€299.50*
2500 units
€1,349.50*

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Selected Catalog #: HSE0101
Product information "ALLin™ Hot Start Taq Polymerase"
highQu ALLin™ Hot Start Taq Polymerase is the superior sensitive hot-start DNA polymerase. The enzyme activity at room temperature is blocked by a small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation occurs and no background appears.
In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors.
For the maximum convenience the ALLin™ Hot Start Taq Mastermix, 2X is available.

Related Products

ALLin™ Red Taq Mastermix
highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors. ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.

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25 mM dNTP Mix
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