phi29 DNA Polymerase

Applications

  • Isothermal DNA amplification for sequencing, cloning
  • RCA – rolling cycle amplification
  • MDA – multiple displacement amplification
  • WGA - whole genome amplification
  • Protein - primed or RNA-primed DNA amplification

Benefits

  • High yield DNA amplification at constant 30°C temperature
  • Robust polymerase with strong strand displacement activity
  • High processivity, synthesis of over 70 kb long DNA strains
  • High fidelity, low error rate for sequencing and cloning
  • Classical enzyme/buffer formulation for known phi29 applications

€159.50*

Product sizes
1000 units
€159.50*
5000 units
€675.50*

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Selected Catalog #: IDE0101
Product information "phi29 DNA Polymerase"

Recombinant phi29 DNA Polymerase is a classical enzyme dedicated for use in common isothermal DNA amplification applications that are carried out at moderate temperature based on a strand displacement activity.

The enzyme is supplied with an optimized high-performance buffer. The user has to add dNTPs, template and primers. phi29 DNA Polymerase was discovered and characterized by M. Salas.

The Polymerase has strong strand displacement activity and efficient 5’-3’ polymerase activity working at about 25-37°C and synthesizing DNA from minor amounts to enormous yield up to visibly increased the viscosity of the reaction mixture.

The enzyme has no 5’-3’ exonuclease activity, but has strong 3’ - 5’ exonuclease (proofreading) activity, and may degrade primers, therefore the use of 3’ protected exo-resistant primers is recommended in the literature. The enzyme can be heat-inactivated, tolerates dUTP and produces blunt-ended DNA.

Most important technical characteristics of phi29 DNA Polymerase (based on abundantly available scientific literature):

  • Strong strand displacement activity
  • 5’-3’ polymerase activity on DNA templates (and on RNA templates)
  • Strong 3’ - 5’ (proofreading) exonuclease activity, may degrade unprotected primers
  • No 5’ - 3’ exonuclease activity
  • Optimal reaction temperature is 30°C
  • Working temperature range is 25-37°C, depends on application
  • Optimal reaction time depends on application
  • The enzyme is inactivated in 10 minutes at 65°C.
  • Can extend both DNA and RNA primers
  • Addition of pyrophosphatase may accelerate the reaction

The use of this product in certain applications, some additives or protocols may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases.

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