highQu supports Coronavirus research and development of SARS-CoV-2 detection tests

 

highQu qPCR and one-step RT-qPCR master mixes of premium quality guarantee reliable and reproducible results in many laboratories worldwide.

 - highQu one-step RT-qPCR kits are already successfully used in Coronavirus detection tests
 - Our production capacities reach 500 000 to 1 million reactions daily
 - One-step RT-qPCR kits are available in bulk quantities from 5000 to 50000 reactions
 - Inquire 24/7 at info@highqu.com  or call +4915786002146
 - Download the COVID-19 detection reagent offers in pdf format

This page combines all information about what highQu offers, per WHO Coronavirus disease guidance and CDC designed RT-PCR assays,
to support all laboratories and companies involved in the development and production of COVID-19 / SARS-CoV-2 testing solutions.

Coronavirus

About the Pandemic situation and Coronavirus testing solutions 

Testing for 2019-nCoV in humans can be briefly divided into three separate stages where different techniques and reagents are used. The first stage is Specimen collection using flocked long nasopharyngeal swabs and specimen transportation in a virus preserving media. The second step is Viral RNA extraction which is in most of the cases performed using automated solutions that employ magnetic bead-based RNA extraction kits. The final testing stage is reverse transcription of RNA and cDNA amplification using virus-specific probes and quantitative One-Step RT qPCR kits. These normally include Reverse transcription mixes and qPCR mixes.

Regretfully, each of these three stages worldwide currently suffers from a shortage of crucial materials and chemicals used to produce all mentioned reagent kits.  This shortage may cause in more people suffering from COVID-19 infection. As a molecular biology reagent supplier, even if not developing coronavirus detection kits itself, highQu strives to support diagnostic companies and laboratories working on kits for SARS-CoV-2 detection. From many companies worldwide we receive daily requests to supply coronavirus testing relevant reagents, such as ready-to-use one-step RT qPCR kits, Reverse Transcriptase, qPCR master mixes, as well as dNTPs. Therefore, we present all our COVID-19-testing related reagents for our OEM customers here in one place.

Produced in bulks under stringent quality requirements in ISO-certified production facilities, our RT-PCR solutions, as well as compound reagents such as dNTPs, will be a great support to diagnostics companies experienced enormous reagent shortage.

Inquire 24/7  info@highqu.com +4915786002146

 

Why shall you order highQu reagents to be used for COVID-19 research and assay development

Your Advantages:

  • - highQu one-step RT-qPCR kits and qPCR master mixes are already successfully used in Coronavirus detection tests
  • - highQu reagents are trusted by laboratories worldwide (publications)
  • - highQu reagents are optimized for fast protocols
  • - Bulk orders are shipped within 4-8 workdays
  • - Produced under stringent quality requirements in ISO-certified facilities
  • - You’ll get 24/7 service @ +4915786002146 and info@highQu.com

 

Sales Terms

  • - To minimize the reagent shortage for life-saving COVID-19 tests, in this emergency we operate under the rule “First come, first served”.
  • - We are committed to delivering the products as soon as possible, however, due to possible logistic/ transport and customs difficulties worldwide, shipments may be delayed.

 

COVID-19 related products in focus - highQu RT qPCR Kits, available in 5 / 50 / 500 ml scale, with 5 to 10 liters daily capacity

Best features:

  • - Robust – inhibitor-resistant, work under a broad range of reaction conditions
  • - Fast - designed for fast-cycling protocols, which enables fast test results
  • - Outperforming – great results on complex templates, and in multiplexing

1Step RT qPCR kits for a single step one tube RT qPCR from RNA templates include qPCR master mix and a blend of thermostable HighScriber™ Reverse Transcriptase with RNase Inhibitor. Kits can be supplied with water. For OEM customers, the kits can be supplied as they are or as separate components. Each component of the kit is available in 5 ml, 50 ml, 500 ml scale. 1Step RT qPCR Probe Kit versions with low or high ROX concentration are also available.

Download Product Insert 1Step RT qPCR Probe Kit

Product and component listing for one-step RT qPCR:

  • #QOP01 – 1Step RT qPCR Probe Kit
  • #QOP02 – 1Step RT qPCR Probe ROX L Kit
  • #QOP03 – 1Step RT qPCR Probe ROX H Kit
  • #QOP01c1 – 1Step RT qPCR Probe Mix, 2X – qPCR master mix optimized for one-step RT qPCR, a component of #QOP01.
  • #QOP02c1 – 1Step RT qPCR Probe Mix, 2X – qPCR master mix optimized for one-step RT qPCR, a component of #QOP02.
  • #QOP03c1 – 1Step RT qPCR Probe Mix, 2X – qPCR master mix optimized for one-step RT qPCR, a component of #QOP03.
  • #QOP01c2 – RT3 Mix, 20X – RT enzyme blend, a component of the above-mentioned kits.
  • #WAT01 – PCR Water, a component of the above-mentioned kits.

 

Inquire 24/7  info@highqu.com +4915786002146

 

COVID-19 related products - highQu qPCR master mixes, available in 5 / 50 / 500 ml scale

Best features:

  • - Robust – inhibitor-resistant, work under a broad range of reaction conditions
  • - Fast - designed for fast-cycling protocols, which enables fast test results
  • - Outperforming – great results on complex templates, and in multiplexing

qPCR master mixes (can be combined in your own 1Step RT qPCR Kits) allow to achieve the highest sensitivity under both standard and fast cycling conditions and provide excellent results on complex templates with minimum or no optimization.

Download Product Insert ORA™ qPCR Probe Mix, 2X

Listing of qPCR Master mixes:

 

Inquire 24/7  info@highqu.com +4915786002146

 

COVID-19 related basic kit components in focus - highQu dNTPs available in 1 ml, 25 ml, 100 ml, and larger scale

Best features:

  • - ≥99% HPLC-confirmed purity
  • - Pure from contaminating & deaminated nucleotides, pyrophosphates
  • - Free from nucleases & proteases, from bacterial & human DNA
  • - Functionally tested in demanding applications
  • - Stable during storage/usage & multiple freezing/thawing

 

highQu dNTPs Product Listing:

  • #NUCA dATP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml, and larger scale.
  • #NUCC dCTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml, and larger scale.
  • #NUCG dGTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml, and larger scale.
  • #NUCT dTTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml, and larger scale.

 

Inquire 24/7  info@highqu.com +4915786002146

 

Other Products useful for COVID-19 research and development of detection kits

 

Last but not least…

We know you know how it works and how to optimize your reactions… Nevertheless, take a minute to check our best PCR optimization tips for faster results in our OEM customer’s hands:

1. Tip number one - MIXING of all reagents is very important for your qPCR results

Do not underestimate the importance of mixing of all the reagents before use. It is especially important when working with bulk quantities and concentrated solutions. Highly concentrated dNTPs or buffers used without thorough mixing provide inconsistency in results due to concentration fluctuations.

2. Tip number two – optimization of CYCLING conditions is a key

Every reagents supplier suggests running the annealing temperature gradient for your first qPCR experiment with a new master mix or a new primer-probe-template set. Please, always do it.

Different buffers’ salt concentrations (ionic strengths) change the annealing behavior of your primers and probes. Each new qPCR master mix requires cycling adjustments.

highQu qPCR master mixes are of high ionic strengths and require higher annealing temperatures for the most optimal performance.

Start your experiment with highQu qPCR mixes by applying annealing gradient with 0,5-degree increments in a range of 58 ºC to 65 ºC. The cycling times may also differ from mix to mix. Do not perform too long denaturation, elongation or annealing steps, follow supplier’s recommendations first.

 

Enjoy our VIDEO summary of all said above and contact us if we can help.

Inquire 24/7  info@highqu.com +4915786002146