ORA™ SEE qPCR Probe Mix, 2X

Applications


  • qPCR assays based on specific probes:

  • TaqMan®, Molecular Beacons, Scorpions™ Probes

  • Gene expression analysis, quantification of:

  • gDNA, cDNA, viral DNA, low copy number genes

Benefits


  • Universal - both standard and fast cycling, all probe qPCR assays

  • qPCR of GC or AT rich templates, single-plex & multiplexing

  • Rapid extension, early Ct

  • Inert blue dye for a better sample visibility and tracking

  • Bulk quantities & custom packaging available

Catalog. # Product Name Size Price Qty
QPP0401 ORA™ SEE qPCR Probe Mix, 2X 200 r of 20 µl
€117.50
QPP0405 ORA™ SEE qPCR Probe Mix, 2X 1000 r of 20 µl
€529.50

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Description

highQu qPCR mastermixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization.

ORA™ SEE qPCR mixes provide an additional advantage of a simplified tracking of the process, as they are colored with an inert blue dye to make samples much better visible during pipetting and handling.

Our mastermixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Mastermixes are available in three versions – without ROX, with low or high ROX concentration.

Protocols

Print version of the protocol: Product Insert ORA™ SEE qPCR Probe Mix, 2X
Important Notes
  • Use special primer selection programs for good planning.
  • Work with amplicons in a range of 80-200, max 400 bp.
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Run reactions in triplets; include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.
 
Prepare a 20 µl reaction:
Reverse Primer 100 - 400 nM final c.
Forward Primer 100 - 400 nM final c.
Specific Probe 200 nM final c.
cDNA Template or
gDNA Template
<100 ng or
<1 μg
PCR Water to 10 μl
ORA™ qPCR Mix, 2X 10 μl

 

  • Mix gently, avoid bubbles.
  • Place into the instrument set like:

 

Initial denaturation 1 cycle: 95°C - 2 min for cDNA  or
1 cycle: 95°C - 3 min for gDNA
Denaturation 40 cycles: 95°C - 5 sec
Anneal./extension 40 cycles: 60-65°C - 20-30 sec

 

  • Follow instrument instructions for melt curve analysis.

Specifications

CAT. SIZE COMPONENTS COMPOSITION
QPP0401 200 r
of 20 µl
2 x 1 ml - ORA™ SEE qPCR Probe Mix, 2X
2 x 1 ml - PCR Water
Hot Start qPCR components: dNTPs at 0.25 mM, optimized buffer, ROX is not included, inert blue dye.
QPP0405 1000 r
of 20 µl
10 x 1 ml - ORA™ SEE qPCR Probe Mix, 2X
10 x 1 ml - PCR Water
Hot Start qPCR components: dNTPs at 0.25 mM, optimized buffer, ROX is not included, inert blue dye.

 

Storage

 

In the dark at -20°C.

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