ORA™ qPCR HRM Mix, 2X

Applications


  • High Resolution Melting analysis (HRM):

  • Detection of sequence variations

  • SNP genotyping

  • Methylation analysis

  • Mutation scanning

Benefits


  • Time and costs saving analysis of sequence variations

  • Universal - standard or fast cycling, GC or AT rich templates

  • Highest sensitivity, no optimization required

  • Supplied with PCR Water for maximum convenience

  • Bulk quantities & custom packaging available

Catalog. # Product Name Size Price Qty
QPD0301 ORA™ qPCR HRM Mix, 2X 200 r of 20 µl
CHF142.18
QPD0305 ORA™ qPCR HRM Mix, 2X 1000 r of 20 µl
CHF640.70

ORA™ qPCR HRM Mix, 2X

Request a Sample.

Description

High Resolution Melting analysis (HRM) is a fast and simple technique for identification of DNA sequence variations. It allows identifying single nucleotide differences by detecting minor changes in qPCR melting curves.

highQu ORA™ HRM qPCR Mix includes a proprietary intercalating saturating dye showing no inhibition for PCR. The dye has the same affinity for both AT or GC rich sequences what leads to highest accuracy in genotyping.

The hot-start function in the mix is based on the small molecular inhibitor technology and allows achieving highest sensitivity and specificity under both standard and fast qPCR cycling conditions. The mix provides excellent performance on both AT and GC rich templates and reliable results with minimum or no optimization.

Our mastermixes are supplied with PCR Water to guaranty the best performance.

Protocols

Print version of the protocol: Product Insert ORA™ qPCR HRM Mix, 2X
Important Note
  • Use special primer selection programs for good planning.
  • Work with amplicons in a range of 80-200, max 400 bp.
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Run reactions in triplets; include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.
  • Do not perform annealing/extension for more than 30 seconds and do not use lower than 60 °C temperature for this step.

 

Prepare a 20 µl reaction:

 

Reverse Primer 100 - 400 nM final c.
Forward Primer 100 - 400 nM final c.
cDNA Template or
gDNA Template
<100ng  or
<1 μg
PCR Water to 10 μl
ORA™ qPCR HRM Mix, 2X 10 μl

 

      • Mix gently, avoid bubbles.
      • Place into the instrument (SYBR® Green or FAM channel), set like:

 

Initial denaturation 1 cycle: 95°C - 2 min for cDNA, or
1 cycle: 95°C - 3 min for gDNA
Denaturation 40 cycles: 95°C - 5 sec
Annealing/extension 40 cycles: 60-65°C - 20-30 sec

 

    • Follow instrument instructions for melt curve analysis.

Specifications

CAT.

SIZE

COMPONENTS

COMPOSITION

QPD0301 200 r of 20 µl 2 x 1 ml - ORA™ qPCR HRM Mix, 2X
2 x 1 ml - PCR Water
Hot Start qPCR components: dNTPs at 0.25 mM, optimized buffer, proprietary saturating intercalating dye.
QPD0305 1000 r of 20 µl 10 x 1 ml - ORA™ qPCR HRM Mix, 2X
10 x 1 ml - PCR Water
Hot Start qPCR components: dNTPs at 0.25 mM, optimized buffer, proprietary saturating intercalating dye.


Storage


In the dark at -20°C.

Resources

Product Insert