ORA™ SEE qPCR Green ROX L Mix, 2X

Applications


  • Dye based qPCR on instruments calibrated with low ROX concentration

  • qPCR from gDNA, cDNA, viral DNA, low copy number genes

  • Relative gene expression analysis, absolute quantification

Benefits


  • Universal - both standard and fast cycling

  • Excellent for GC or AT rich templates

  • Highest sensitivity, rapid extension, early Ct values

  • Inert blue dye for a better sample visibility and tracking

  • Bulk quantities & custom packaging available

Catalog. # Product Name Size Price Qty
QPD0501 ORA™ SEE qPCR Green ROX L Mix, 2X 200 r of 20 µl
€117.50
QPD0505 ORA™ SEE qPCR Green ROX L Mix, 2X 1000 r of 20 µl
€529.50

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Description

highQu qPCR mastermixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization.

ORA™ SEE qPCR mixes provide an additional advantage of a simplified tracking of the process, as they are colored with an inert blue dye to make samples much better visible during pipetting and handling.

Our mastermixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Green Mastermixes are available in different versions –with low or high ROX concentration.

 

For optional use, the ROX passive reference dye is premixed within the ROX L and ROX H qPCR Mixes. If the purchaser has an instrument capable of optional ROX detection and wishes to perform the optional normalization of the signal, then the user must select the option in the software.

Notice to Purchaser: With purchasing of this product, no rights are conveyed with respect to U.S. Patent: 5,928,907 and corresponding patents outside the US.

Protocols

Print version of the protocol: Product Insert ORA™ SEE qPCR Green ROX L Mix, 2X
Important Notes
  • Use special primer selection programs for good planning.
  • Work with amplicons in a range of 80-200, max 400 bp.
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Run reactions in triplets; include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.

 

Prepare a 20 µl reaction:

 

Reverse Primer 100 - 400 nM final c.
Forward Primer 100 - 400 nM final c.
cDNA Template or
gDNA Template
<100ng  or
<1 μg
PCR Water to 10 μl
ORA™ qPCR Mix, 2X 10 μl

 

  • Mix gently, avoid bubbles.
  • Place into the instrument (SYBR® Green or FAM channel), set like:

 

Initial denaturation 1 cycle: 95°C - 2 min for cDNA, or
1 cycle: 95°C - 3 min for gDNA
Denaturation 40 cycles: 95°C - 5 sec
Annealing/extension 40 cycles: 60-65°C - 20-30 sec

 

  • Follow instrument instructions for melt curve analysis.

Specifications

CAT.

SIZE

COMPONENTS

COMPOSITION

QPD0501 200 r
of 20 µl
2 x 1 ml - ORA™ SEE qPCR Green ROX L Mix, 2X
2 x 1 ml - PCR Water
Hot Start qPCR components: dNTPs at 0.25 mM, optimized buffer, low ROX concentration, inert blue dye.
QPD0505 1000 r
of 20 µl
10 x 1 ml - ORA™ SEE qPCR Green ROX L Mix, 2X
10 x 1 ml - PCR Water
Hot Start qPCR components: dNTPs at 0.25 mM, optimized buffer, low ROX concentration, inert blue dye.

 

Storage

 

In the dark at -20°C.

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