End-point PCR Selection Guide

Download NEW Tips and schemes for evaluating of new PCR reagents

Standard & Direct PCR

Product

Specialties

ALLin™ buffer
with dNTPs
Fast cycling GC/AT rich PCR Max product High Yields Direct PCR Multiplex PCR Direct gel loading
SampleIN™ Direct PCR Kit Fast, direct crude sample PCR, includes ALLin™ HS Taq DNA Polymerase in 2X mastermix with red loading dye (available separately)   ●  ●    ●  5 kb  ●  ●●●  ●  ● 
ALLin™ Taq DNA Polymerase, 5 u/µl Robust Taq with ALLin™ buffer for standard, fast, GC rich PCR 6 kb ●● ●   
ALLin™ Red Taq Mastermix, 2X ALLin™ Taq DNA Polymerase in 2X mastermix with loading dye   ●  6 kb   ●●  ●  ● 
ALLin™ Taq Mastermix, 2X ALLin™ Taq DNA Polymerase in 2X mastermix 6 kb ●● ●  ●   
Taq DNA Polymerase, 5 u/µl Classical Taq, magnesium supplied separately       5 kb      

 

Hot Start PCR

Product

Specialties

ALLin™ buffer
with dNTPs
Fast Cycling GC/AT rich PCR High Sensitivity High Yields Fidelity
vs Taq
Product
size
Direct
PCR
Multiplex PCR Cloning
ALLin™ Hot Start Taq Polymerase, 5 u/µl Low copy detection, fast, GC rich, direct PCR ●● ●● 1X 6 kb ●● ●● TA
ALLin™ Hot Start Taq Mastermix, 2X ALLin™ Hot Start Taq in 2X mastermix ●● 1X 6 kb ●●  ●● TA
ALLin™ HS Red Taq Mastermix, 2X ALLin™ Hot Start Taq in 2X mastermix with Red dye for direct gel loading ●● 1X 6 kb ●●● ●● TA
ALLin™ RPH Polymerase, 5 u/µl Robust, Proofreading, Hot-start Polymerase for long PCR ●● ~ 5X 35 kb ●● ●● TA
ALLin™ RPH Mastermix, 2X ALLin™ RPH Polymerase in 2X mastermix ~ 5X 35 kb ●● ●●  TA

 

High Fidelity and Long PCR

Product Specialties ALLin™ buffer
with dNTPs
Fast
cycling
GC/AT rich PCR Sensitive, hot-start Fidelity
vs Taq
Max product Direct
PCR
High Yields Multiplex PCR Cloning
ALLin™ Mega HS HiFi DNA Polymerase, 2 u/µl Hot start version of ALLin™ Mega HiFi Polymerase:
100X higher fidelity than Taq for a very

fast, complex, long PCR, excellent for NGS
~100X >20 kb Blunt
ALLin™ Mega HS HiFi Mastermix, 2X ALLin™ Mega HS HiFi Polymerase in 2X mastermix format ~100X >20 kb Blunt
ALLin™ Mega HS HiFi Red Mastermix, 2X ALLin™ Mega HS HiFi Polymerase in 2X ready-to-load red mastermix format ~100X >20 kb Blunt
ALLin™ Mega HiFi DNA Polymerase, 2 u/µl 100X higher fidelity than Taq for a very
fast, complex, long PCR, excellent for NGS
●● ●●●   ~100X >20 kb ●● Blunt
ALLin™ Mega HiFi Mastermix, 2X ALLin™ Mega HiFi Polymerase in 2X mastermix format ●● ●●●   ~100X >20 kb ●● Blunt
ALLin™ Mega HiFi Red Mastermix, 2X ALLin™ Mega HiFi Polymerase in 2X ready-to-load red mastermix format ●● ●●●   ~100X >20 kb ●● Blunt
ALLin™ HiFi DNA Polymerase, 2 u/µl 50X higher fidelity than Taq for
Fast, GC rich, direct PCR with high fidelity
●●   ~50X 10 kb   Blunt
ALLin™ RPH Polymerase, 5 u/µl Robust Proofreading Hot-start Polymerase for long hot start PCR  ● ●● ~ 5X 35 kb ●● ●● TA
ALLin™ RPH Mastermix, 2X ALLin™ RPH Polymerase in 2X mastermix format  ● ●● ~ 5X 35 kb ●● ●● TA

 

Nucleotides and Other PCR Reagents

Product Specialties
PCRbeam™ Fast PCR Detection Kit Sensitive detection of specific amplification products. The detection is based on immunological reaction driven by Biotin and FITC
UDGin™ PCR Cleaner Mix, 20X An efficient tool for carry-over contamination prevention in qPCR or PCR. Ready to use 20X mix of optimally combined Uracil-DNA Glycosylase (UDG) and dUTP in a buffer
100 mM dNTP Set dNTP Set (includes four separate dNTPs at 100 mM concentration each) for PCR and other molecular biology applications
25 mM dNTP Mix dNTP Mix containing 25 mM concentration of each dNTP in one tube. Can be used as 100X dNTP working solution for PCR where 0.25 mM final dNTP concentration is required
10 mM dNTP Mix dNTP Mix containing 10 mM concentration of each dNTP in one tube. Can be used as 50X dNTP working solution for PCR where 0.2 mM final dNTP concentration is required
PCR Water Pure PCR-grade, nuclease free water for all molecular biology applications