ALLin™ Taq Mastermix, 2X

Applications


  • Routine PCR up to 6 kb

  • PCR of GC rich templates

  • Colony PCR, Fast PCR, TA cloning

Benefits

Catalog. # Product Name Size Price Qty
PCM0101 ALLin™ Taq Mastermix, 2X 200 r of 50 µl
€99.50
PCM0105 ALLin™ Taq Mastermix, 2X 1000 r of 50 µl
€449.50

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Description

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in  demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 104 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors.

The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.

Protocols

Print version of the protocol: Product Insert ALLin™ Taq Mastermix, 2X
Important Notes
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.
  • The longer the amplicon, the longer the extension time: 15 sec/kb - <6kb and 1 sec/kb - <1kb. Use >90 sec extension for multiplexing
  • Run an annealing temperature gradient from 55°C to 65°C to choose the best specificity conditions.
  • Do not use fast cycling for multiplexing.
 
Prepare a 50 µl PCR reaction

 

Rev. & For. Primers variable, up to 0.4 µM final each (2 µl of 10 µM each)
cDNA Template  or
gDNA Template
<100 ng  or
5 - 500 ng
PCR Water to 25 μl
ALLin™ Taq Mastermix, 2X 25 µl

 

  • Mix gently, avoid bubbles.
  • Place into the instrument set like:

 

Initial denaturation 1 cycle: 95°C - 1 min
Denaturation 40 cycles: 95°C - 15 sec
Annealing 40 cycles: 55-65°C - 15 sec
Extension 40 cycles: 72°C – 1 - 90 sec (15 sec/kb)

 

  • After the PCR store probes on ice shortly, store at -20°C for long term.

Specifications

Cat.

Size

Components

Composition

PCM0101 200 r of
50 µl
5 x 1 ml - ALLin™ Taq Mastermix, 2X
5 x 1 ml - PCR Water
1X mastermix contains 0.25 mM dNTPs, 3 mM MgCl2, enhancers, stabilizers.
PCM0105 1000 r of
50 µl
25 x 1 ml - ALLin™ Taq Mastermix, 2X
25 x 1 ml - PCR Water
1X mastermix contains 0.25 mM dNTPs, 3 mM MgCl2, enhancers, stabilizers.


Storage


  In the dark at -20°C.

Resources

 

Product Insert