ALLin™ Red Taq Mastermix, 2X

Applications


  • Routine PCR up to 6 kb with a direct gel loading option

  • PCR of GC rich templates

  • Colony PCR, Fast PCR, TA cloning

Benefits

Catalog. # Product Name Size Price Qty
PCM0201 ALLin™ Red Taq Mastermix, 2X 200 r of 50 µl
€99.50
PCM0205 ALLin™ Red Taq Mastermix, 2X 1000 r of 50 µl
€449.50

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Description

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in  demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 104 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors.

The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors.

ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR.

In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.

The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.

Protocols

Print version of the protocol: Product Insert ALLin™ Red Taq Mastermix, 2X
Important Notes
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.
  • The longer the amplicon, the longer the extension time: 15 sec/kb - <6kb and 1 sec/kb - <1kb. Use >90 sec extension for multiplexing
  • Run an annealing temperature gradient from 55°C to 65°C to choose the best specificity conditions.
  • Do not use fast cycling for multiplexing.
 
Prepare a 50 µl PCR reaction

 

Rev. & For. Primers variable, up to 0.4 µM final each (2 µl of 10 µM each)
cDNA Template  or
gDNA Template
<100 ng  or
5 - 500 ng
PCR Water to 25 μl
ALLin™ Red Taq Mastermix, 2X 25 µl

 

  • Mix gently, avoid bubbles.
  • Place into the instrument set like:

 

Initial denaturation 1 cycle: 95°C - 1 min
Denaturation 40 cycles: 95°C - 15 sec
Annealing 40 cycles: 55-65°C - 15 sec
Extension 40 cycles: 72°C – 1 - 90 sec (15 sec/kb)

 

  • Load probes on the agarose gel. The red loading dye is included in the mastermix.

  • ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.

  • Store probes on ice shortly, store at -20°C for long term.

Specifications

Cat.

Size

Components

Composition

PCM0201 200 r of
50 µl
5 x 1 ml - ALLin™ Red Taq Mastermix, 2X
5 x 1 ml - PCR Water
1X mastermix contains 0.25 mM dNTPs, 3 mM MgCl2, enhancers, stabilizers, red electrophoresis tracking dye and density reagents for gel loading
PCM0205 1000 r of
50 µl
25 x 1 ml - ALLin™ Red Taq Mastermix, 2X
25 x 1 ml - PCR Water
1X mastermix contains 0.25 mM dNTPs, 3 mM MgCl2, enhancers, stabilizers, red electrophoresis tracking dye and density reagents for gel loading


Storage


  In the dark at -20°C.

Resources

 

Product Insert