Description
highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions.
Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations.
Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature storage or long PCR of more than 30 kb targets (see the picture of Long PCR obtained with highQu dNTPs) as well as long amplifications exceeding 40 cycles.
Protocols
Print version of the protocol: Product Insert 25 mM dNTP Mix
Protocol recommendations for standard PCR
- Typical concentration of each dNTP in the PCR reaction is 0.2 – 0.25 mM.
- Higher concentration increase yields, however Mg2+ ions bind to dNTPs, therefore, both components shall be present in coordinated concentrations.
- Too high dNTPs and magnesium concentrations reduce PCR fidelity.
- Mix well each dNTP and magnesium solution, to avoid concentration fluctuations.
- We recommend final 3 mM MgCl2 with 0.25 mM each dNTP concentration for routine PCR.
- Recommended volumes of dNTP Mixes and 50 mM MgCl2 are given below, however those are just guidelines, and each reaction might require optimization.
| Starting dNTP Mix conc. | Vol. of dNTP mix for 50 µl rxn to achieve 0.25 mM concentration of each dNTP |
| 10 mM | 1.25 µl |
| 25 mM | 0.5 µl |
| Final Mg2+ conc. in rxn | Vol. of 50 mM MgCl2 for 50 µl rxn to achieve desired conc. |
| 2 mM | 2 µl |
| 3 mM | 3 µl |
Specifications
| CAT. | SIZE/CONTENT | COMPOSITION | |
| NUM0101 | 1 ml - 25 mM dNTP Mix | Aqueous solution of equal concentration of 25 mM of each of 4 dNTPs, pH 8.5 (at 4°C) |
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Storage |
In the dark at -20°C. |
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